blue ap substrate kit Search Results


dab substrate  (Vector Laboratories)
96
Vector Laboratories dab substrate
Dab Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dab substrate/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
dab substrate - by Bioz Stars, 2026-05
96/100 stars
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ap conjugate substrate kit  (Bio-Rad)
99
Bio-Rad ap conjugate substrate kit
Ap Conjugate Substrate Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ap conjugate substrate kit/product/Bio-Rad
Average 99 stars, based on 1 article reviews
ap conjugate substrate kit - by Bioz Stars, 2026-05
99/100 stars
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blue alkaline phosphatase substrate kit  (Vector Laboratories)
96
Vector Laboratories blue alkaline phosphatase substrate kit
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Blue Alkaline Phosphatase Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/blue alkaline phosphatase substrate kit/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
blue alkaline phosphatase substrate kit - by Bioz Stars, 2026-05
96/100 stars
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p nitrophenyl phosphate  (Bio-Rad)
93
Bio-Rad p nitrophenyl phosphate
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
P Nitrophenyl Phosphate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p nitrophenyl phosphate/product/Bio-Rad
Average 93 stars, based on 1 article reviews
p nitrophenyl phosphate - by Bioz Stars, 2026-05
93/100 stars
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black substrate kit alkaline phosphatase  (Vector Laboratories)
95
Vector Laboratories black substrate kit alkaline phosphatase
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Black Substrate Kit Alkaline Phosphatase, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/black substrate kit alkaline phosphatase/product/Vector Laboratories
Average 95 stars, based on 1 article reviews
black substrate kit alkaline phosphatase - by Bioz Stars, 2026-05
95/100 stars
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bromo 4chloro 3 indolyl phosphate nitro blue tetrazolium substrate kit  (Vector Laboratories)
94
Vector Laboratories bromo 4chloro 3 indolyl phosphate nitro blue tetrazolium substrate kit
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Bromo 4chloro 3 Indolyl Phosphate Nitro Blue Tetrazolium Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bromo 4chloro 3 indolyl phosphate nitro blue tetrazolium substrate kit/product/Vector Laboratories
Average 94 stars, based on 1 article reviews
bromo 4chloro 3 indolyl phosphate nitro blue tetrazolium substrate kit - by Bioz Stars, 2026-05
94/100 stars
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fast red substrate kit  (Nichirei Corporation)
90
Nichirei Corporation fast red substrate kit
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Fast Red Substrate Kit, supplied by Nichirei Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fast red substrate kit/product/Nichirei Corporation
Average 90 stars, based on 1 article reviews
fast red substrate kit - by Bioz Stars, 2026-05
90/100 stars
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ap substrate kit  (Burlington Industries)
90
Burlington Industries ap substrate kit
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Ap Substrate Kit, supplied by Burlington Industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ap substrate kit/product/Burlington Industries
Average 90 stars, based on 1 article reviews
ap substrate kit - by Bioz Stars, 2026-05
90/100 stars
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ap chemiluminescent substrate tropix kit  (Tropix Inc)
90
Tropix Inc ap chemiluminescent substrate tropix kit
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Ap Chemiluminescent Substrate Tropix Kit, supplied by Tropix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ap chemiluminescent substrate tropix kit/product/Tropix Inc
Average 90 stars, based on 1 article reviews
ap chemiluminescent substrate tropix kit - by Bioz Stars, 2026-05
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ap-substrate-kit  (Linaris GmbH)
90
Linaris GmbH ap-substrate-kit
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Ap Substrate Kit, supplied by Linaris GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ap-substrate-kit/product/Linaris GmbH
Average 90 stars, based on 1 article reviews
ap-substrate-kit - by Bioz Stars, 2026-05
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horseradish peroxidase labelled secondary antibodies and alp or peroxidase substrate solutions vectastain® abc-ap staining kit  (Linaris GmbH)
90
Linaris GmbH horseradish peroxidase labelled secondary antibodies and alp or peroxidase substrate solutions vectastain® abc-ap staining kit
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Horseradish Peroxidase Labelled Secondary Antibodies And Alp Or Peroxidase Substrate Solutions Vectastain® Abc Ap Staining Kit, supplied by Linaris GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/horseradish peroxidase labelled secondary antibodies and alp or peroxidase substrate solutions vectastain® abc-ap staining kit/product/Linaris GmbH
Average 90 stars, based on 1 article reviews
horseradish peroxidase labelled secondary antibodies and alp or peroxidase substrate solutions vectastain® abc-ap staining kit - by Bioz Stars, 2026-05
90/100 stars
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vector sg peroxidase (hrp) substrate kit  (Vector Laboratories)
96
Vector Laboratories vector sg peroxidase (hrp) substrate kit
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Vector Sg Peroxidase (Hrp) Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vector sg peroxidase (hrp) substrate kit/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
vector sg peroxidase (hrp) substrate kit - by Bioz Stars, 2026-05
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Image Search Results


Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline phosphatase-stained colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.

Journal: bioRxiv

Article Title: Replicable generation of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia

doi: 10.64898/2026.03.17.712482

Figure Lengend Snippet: Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline phosphatase-stained colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.

Article Snippet: After 5 days, colonies were stained with a Vector Blue Alkaline Phosphatase Substrate kit (Vector Laboratories, cat. no. SK-5300) as described in .

Techniques: Cloning, Staining, Cell Culture

Characterization of RhiPSC line W6 clone 5.07. (A) Amplicon sequencing of MAPT R406W with wild-type C allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P16, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 94.5% positive for SOX2, 99.2% positive for OCT4, and 95% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P12. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

Journal: bioRxiv

Article Title: Replicable generation of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia

doi: 10.64898/2026.03.17.712482

Figure Lengend Snippet: Characterization of RhiPSC line W6 clone 5.07. (A) Amplicon sequencing of MAPT R406W with wild-type C allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P16, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 94.5% positive for SOX2, 99.2% positive for OCT4, and 95% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P12. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

Article Snippet: After 5 days, colonies were stained with a Vector Blue Alkaline Phosphatase Substrate kit (Vector Laboratories, cat. no. SK-5300) as described in .

Techniques: Amplification, Sequencing, Staining, Reverse Transcription Polymerase Chain Reaction, Control, Plasmid Preparation, Positive Control, Immunofluorescence, Flow Cytometry, Virus

Characterization of RhiPSC line M6 clone 16.02. (A) Amplicon sequencing of MAPT R406W with mutant T allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P18, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 93.1% positive for SOX2, 98% positive for OCT4, and 93.3% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P15. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

Journal: bioRxiv

Article Title: Replicable generation of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia

doi: 10.64898/2026.03.17.712482

Figure Lengend Snippet: Characterization of RhiPSC line M6 clone 16.02. (A) Amplicon sequencing of MAPT R406W with mutant T allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P18, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 93.1% positive for SOX2, 98% positive for OCT4, and 93.3% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P15. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

Article Snippet: After 5 days, colonies were stained with a Vector Blue Alkaline Phosphatase Substrate kit (Vector Laboratories, cat. no. SK-5300) as described in .

Techniques: Amplification, Sequencing, Mutagenesis, Staining, Reverse Transcription Polymerase Chain Reaction, Control, Plasmid Preparation, Positive Control, Immunofluorescence, Flow Cytometry, Virus